Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status
نویسندگان
چکیده
منابع مشابه
An evaluation of a modified erythrocyte transketolase assay for assessing thiamine nutritional adequacy.
Transketolase (TK) activity was assessed by incubating hemolyzed whole blood with ribose-5-phosphate both in the presence (TKsat.) and in the absence (TK) of thiamine pyrophosphate (TPP). The amount of pentoses utilized between 5 and 60 min after the start of the incubation was taken as a measure of the TK activity. The TPP effect was expressed at (TKsat. - TK)/TK x 100. The coefficient of vari...
متن کاملCorrespondence: Measurement of erythrocyte transketolase activity
CorreSpondence Measurement of erythrocyte transketolase activity Correspondence from Donald E. King C. R. Milner et al.'s recent paper compared automated versus manual procedures for the 'Measurement of erythrocyte trans-ketolase activity on a discrete analyser' (Journal of Automatic Chemistry, Vol. 4, No. 4, pp. 183-185). In it the author uses regression analysis to evaluate the similarity of ...
متن کاملThe measurement of erythrocyte transketolase activity on a discrete analyser
Transketolase (TK, EC.2.2.1.1) is a thiamine-dependent enzyme which is widely used for assessing thiamine deficiency. The TK activity in erythrocytes is usually decreased during thiamine deficiency and this activity can be enhanced in vitro by the addition of thiamine pyrophosphate (TPP). The relative increase in enzyme activity, expressed as a percentage, is called the TPP effect. The TK kinet...
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چکیده هدف اصلی از طراحی لرزه ای تامین ایمنی جانی در هنگام وقوع زلزله و تعمیر پذیر بودن سازه خسارت دیده، پس از وقوع زلزله است. تجربه زلزله های اخیر نشان داده است که ساختمان های طراحی شده با آیین نامه های مبتنی بر نیرو از نظر محدود نمودن خسارت وارده بر سازه دقت لازم را ندارند. این امر سبب پیدایش نسل جدید آیین نامه های مبتنی بر عملکرد شده است. در این آیین نامه ها بر اساس تغییرشکل های غیرارتجاعی ...
15 صفحه اولEffects of methodological variation on assessment of riboflavin status using the erythrocyte glutathione reductase activation coefficient assay.
Riboflavin status is usually measured as the in vitro stimulation with flavin adenine dinucleotide of the erythrocyte enzyme glutathione reductase, and expressed as an erythrocyte glutathione reductase activation coefficient (EGRAC). This method is used for the National Diet and Nutrition Surveys (NDNS) of the UK. In the period between the 1990 and 2003 surveys of UK adults, the estimated preva...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 2020
ISSN: 0077-8923,1749-6632
DOI: 10.1111/nyas.14547